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Human IGF-I Protein, His Tag (SPR verified)

Human IGF-I Protein, His Tag (SPR verified)
别名: IGF-I,IGF1A,somatomedin C,MGF
储存条件: -20℃ 
Human IGF-I Protein, His Tag (SPR verified)
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-IG1-H5245-100ug 100ug 现货 2900.00 2900
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熔点:
密度:
储存条件: -20℃

Human IGF-I Protein, His Tag (SPR verified)

分子别名(Synonym)

IGF-I,IGF1A,somatomedin C,MGF

表达区间及表达系统(Source)

Human IGF-I, His Tag  is expressed from human 293 cells). It contains AA Gly 49 - Ala 118

Predicted N-terminus: His

Request for sequence

蛋白结构(Molecular Characterization)

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 9.5 kDa. The protein migrates as 12 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

Human IGF-I, His Tag (Cat. No. IG1-H5245) SDS-PAGE gel

Human IGF-I, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

活性(Bioactivity)-SPR

Biotinylated Human SPR

Biotinylated Human IGF-I R, His,Avitag  immobilized on CM5 Chip can bind Human IGF-I, His Tag with an affinity constant of 33.9 nM as determined in a SPR assay (Biacore 8K) (QC tested).

 

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