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别名: | PDGF-BB,PDGF-B,FLJ12858,PDGF2,SIS,SSV,c-sis | ||
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储存条件: | -20℃ |
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货号 | 规格 | 可用库存 | 销售价(RMB) | 您的折扣价(RMB) | 购买数量 |
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熔点: | |
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密度: | |
储存条件: | -20℃ |
Unconjugated Human PDGF-BB Protein, His,Avitag™
PDGF-BB,PDGF-B,FLJ12858,PDGF2,SIS,SSV,c-sis
Unconjugated Human PDGF-BB, His,Avitag is expressed from E.coli cells. It contains AA Ser 82 - Thr 190.
Predicted N-terminus: Met
Request for sequence
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 15.1 kDa. the protein migrates as 17 kDa under reducing (R) condition, and 35 kDa under non-reducing (NR) condition (SDS-PAGE).
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 0.085% TFA in 30% ACN. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Unconjugated Human PDGF-BB, His,Avitag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Unconjugated Human PDGF-BB, His,Avitag at 1 μg/mL (100 μL/well) can bind Human PDGF R beta, Fc Tag with a linear range of 0.5-16 ng/mL (QC tested).