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Biotinylated Mouse CD27 Ligand / CD70 Protein, His,Avitag™

Biotinylated Mouse CD27 Ligand / CD70 Protein, His,Avitag™
别名: CD70,CD27LG,TNFSF7,TNFSF7G,CD27L
储存条件: -20℃ 
Biotinylated Mouse CD27 Ligand / CD70 Protein, His,Avitag™
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-CDL-M82Qb-25ug 25ug 现货 3500.00 3500
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熔点:
密度:
储存条件: -20℃

Biotinylated Mouse CD27 Ligand / CD70 Protein, His,Avitag™

 

分子别名(Synonym)

CD70,CD27LG,TNFSF7,TNFSF7G,CD27L

表达区间及表达系统(Source)

Biotinylated Mouse CD27 Ligand, His,Avitag  is expressed from human 293 cells. It contains AA Gln 47 - Pro 195.

Predicted N-terminus: His

Request for sequence

蛋白结构(Molecular Characterization)

This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 20.1 kDa. The protein migrates as 28-32 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Biotinylation)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Biotin:Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

Biotinylated Mouse CD27 Ligand, His,Avitag (Cat. No. CDL-M82Qb) SDS-PAGE gel

Biotinylated Mouse CD27 Ligand, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Mouse CD27, Mouse IgG2a Fc Tag, low endotoxin t 2 μg/mL (100 μL/well) can bind Biotinylated Mouse CD27 Ligand, His,Avitag  with a linear range of 0.1-3 ng/mL (QC tested).

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