-
专业包装 正品保证
-
快乐服务 售后无忧
-
会员特权 优惠不断
-
个人信息 严格保护
| 别名: | IL1B,IL-1BETA,IL1F2,IL-1β | ||
|---|---|---|---|
| 储存条件: | -20℃ | ||
|
| 货号 | 规格 | 可用库存 | 销售价(RMB) | 您的折扣价(RMB) | 购买数量 |
|---|
| 熔点: | |
|---|---|
| 密度: | |
| 储存条件: | -20℃ |
Human IL-1 beta / IL-1F2 Protein, His Tag (MALS verified)
IL1B,IL-1BETA,IL1F2,IL-1β
Human IL-1 beta, His Tagis expressed from E.coli cells. It contains AA Ala 117 - Ser 269
Predicted N-terminus: Met
Request for sequence
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 19.3 kDa. The protein migrates as 19-20 kDa under reducing (R) condition (SDS-PAGE).
Less than 1.0 EU per μg by the LAL method.
>95% as determined by reduced SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Human IL-1 beta, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human IL-1 beta, His Tag is more than 90% and the molecular weight of this protein is around 17-22 kDa verified by SEC-MALS.
Report
Immobilized Human IL-1 beta, His Tagat 10 μg/mL (100 μL/well) can bind Human IL-1 RII, Fc Tagwith a linear range of 2-13 ng/mL (QC tested).
Protocol
Human IL-1 RII, Fc Tag captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human IL-1 beta, His Tag with an affinity constant of 0.571 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
