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| 别名: | IL21,Za11,Interleukin-21 | ||
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| 储存条件: | -20℃ | ||
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| 货号 | 规格 | 可用库存 | 销售价(RMB) | 您的折扣价(RMB) | 购买数量 |
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| 熔点: | |
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| 密度: | |
| 储存条件: | -20℃ |
Biotinylated Human IL-21 Protein, Fc,Avitag™ (MALS verified)
IL21,Za11,Interleukin-21
Biotinylated Human IL-21, Fc,Avitag is expressed from human 293 cells . It contains AA Gln 30 - Ser 162.
Predicted N-terminus: Gln 30
Request for sequence
This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 43.6 kDa. The protein migrates as 50-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Passed as determined by the HABA assay / binding ELISA.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Biotinylated Human IL-21, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Biotinylated Human IL-21, Fc,Avitagis more than 85% and the molecular weight of this protein is around 110-130 kDa verified by SEC-MALS.
Report
Immobilized Human IL-21 R, Fc Tagat 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-21, Fc,Avitag with a linear range of 0.2-6 ng/mL (QC tested).
Protocol
Biotinylated Human IL-21, Fc,Avita immobilized on CM5 Chip can bind Human IL-21 R, Fc Tag with an affinity constant of 0.165 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
