Anti-TPA antibody [AH81-00]

Anti-TPA antibody [AH81-00] 

• 概述

• 产品描述uPA (urokinase-type plasminogen activator) and tPA (tissue plasminogen activator), which are serine proteases and members of the trypsin family, are essential to the intrinsic coagulation system. tPA is primarily involved in fibrinolysis, whereas uPA principally mediates cell migration and tissue remodeling processes. uPA and tPA are responsible for cleaving plasminogen, a large serum β-globulin that is deposited on the Fibrin strands within a thrombus. uPA and tPA preferentially target plasminogen at the Arg-Val bond to produce plasmin (also designated fibrinolysin), which is a trypsin-like enzyme that acts on Arg-Lys bonds in Fibrin and Fibrinogen and contributes to the systematic activation of the coagulation cascade. uPA and tPA each consist of two chains that are designated A and B. The A chain of uPA can be cleaved, resulting in low and high molecular mass forms. uPA and tPA are regulated by the serpin family members PAI-1 and PAI-2, which are serine proteinase inhibitors that complex with uPA, tPA and other targeted proteinases and then slowly disassociate to produce cleaved species that fold into stable inactive conformations.
• 产品名称Anti-TPA antibody [AH81-00]
• 分子量62/30 kDa
• 种属反应性Human,Mouse,Rat
• 验证应用ELISA,WB,ICC,IHC-P
• 抗体类型小鼠单抗
• 免疫原recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at -20 ℃. Stable for 12 months from date of receipt.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.22% Sodium Azide.
• 亚型IgG1
• 亚细胞定位Secreted.
• 其它名称
  • Alteplase antibody
  • DKFZp686I03148 antibody
  • Plasminogen activator tissue antibody
  more

• 应用

  WB: 1:1,000-1:5,000
  ICC: 1:50-1:200
  IHC-P: 1:50-1:200
  ELISA: 1,5,000-1:10,000

•  

  

  Fig1: ICC staining TPA in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig2: ICC staining TPA in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig3: ICC staining TPA in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-TPA antibody. Counter stained with hematoxylin.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-TPA antibody. Counter stained with hematoxylin.

   

  

  Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-TPA antibody. Counter stained with hematoxylin.

   

  

  Fig7: Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-TPA Tubulin antibody. Counter stained with hematoxylin.

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