Anti-CD134/OX40 antibody [2H2]

Anti-CD134/OX40 antibody [2H2]

• 概述

• 产品描述Ox40 (also designated CD134 and Ox40R), is a member of the tumor necrosis factor receptor (TNFR) family. Ox40 is involved in coordinating CD4 T cell selection, migration and cytokine differentiation in T helper (Th)1 and Th2 cells. Ox40 is also involved in the stimulation of T cells, T cell-dependent humoral response and generation of optimal CD4+ T cell responses in vivo and in vitro. Ox40 is expressed on activated CD4+ T lymphocytes and its ligand, Ox40L, is found preferentially on activated B cells. Engagement of Ox40 with its ligand, Ox40L, delivers a strong costimulatory signal to effector T cells. Members of the TNFR superfamily are critically involved in the regulation of infections, inflammation, autoimmune diseases and tissue homeostasis.
• 产品名称Anti-CD134/OX40 antibody [2H2]
• 分子量55 kDa
• 种属反应性Human,Mouse,Rat
• 验证应用WB,IHC-P,ICC,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Membrane.
• 其它名称
  • ACT 35 antibody
  • ACT35 antibody
  • ACT35 antigen antibody
  more

• 应用

  ICC: 1:50-1:200
  IHC-P: 1:50-1:200
  FC: 1:50-1:100
  WB: 1:500

•  

  

  Fig1: ICC staining CD134 (green) in A549 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig2: ICC staining CD134 (green) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig3: ICC staining CD134 (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD134 antibody. Counter stained with hematoxylin.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD134 antibody. Counter stained with hematoxylin.

   

  

  Fig6: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-CD134 antibody. Counter stained with hematoxylin.

   

  

  Fig7: Flow cytometric analysis of HL-60 cells with CD134 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti mouse IgG was used as the secondary

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