Anti-LRRK1 antibody [5B1]

Anti-LRRK1 antibody [5B1]

• 概述

• 产品描述Leucine-rich repeat serine/threonine-protein kinase 1 is an enzyme that in humans is encoded by the LRRK1 gene. Formation of LRRK1:EGF-R complexes result in LRRK cellular translocation: LRRK1 traffics together with EGF-R to endosomes upon stimulation of the receptor with EGF. LRRK1 is related to the familial Parkinsonism gene product Park8/LRRK2, and contains a Ras of complex proteins (ROC) GTPase domain and a MAPKKK-like kinase domain. LRRK1 participates in the intercellular trafficking of the epidermal growth factor (EGF) receptor. LRRK1 is activated by the sequential phosphorylation of 2 mitotic kinases, PLK1 and CDK1. Importantly, this PLK1-dependent activation of LRRK1 is required for the regulation of mitotic spindle orientation.LRRK1 regulates spindle orientation in a manner dependent on its kinase activity. Furthermore, the spindle misorientation caused by PLK1 inactivation is rescued by expression of the phospho-mimicking mutant LRRK1(S1790D). LRRK1 regulates spindle orientation downstream of PLK1.
• 产品名称Anti-LRRK1 antibody [5B1]
• 分子量225kDa, additional band 60kDa
• 种属反应性Human,Mouse
• 验证应用WB,IHC-P
• 抗体类型小鼠单抗
• 免疫原Peptide
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG2b
• 纯化方式Peptide affinity purified
• 亚细胞定位Cytoplasm.
• 其它名称
  • KIAA1790 antibody
  • Leucine rich repeat serine/threonine protein kinase 1 antibody
  • Leucine-rich repeat serine/threonine-protein kinase 1 antibody
  more

• 应用

  WB: 1:500
  IHC-P: 1:50-1:100

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  Fig1: Western blot analysis of LRRK1 on A549 lysate using anti-LRRK1 antibody at 1/100 dilution.

   

  

  Fig2: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-LRRK1 antibody. Counter stained with hematoxylin.

   

  

  Fig3: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-LRRK1 antibody. Counter stained with hematoxylin.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-LRRK1 antibody. Counter stained with hematoxylin.

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