Anti-GABRA2 antibody

Anti-GABRA2 antibody

• 概述

• 产品描述GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.

• 产品名称Anti-GABRA2 antibody

• 分子量48 KDa

• 种属反应性Human,Mouse,Rat,Dog,Pig,Cow,Horse,Rabbit

• 验证应用WB,IHC-P,FC

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human GABRA2/GABA A Receptor alpha 2 185-280/451

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.

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• ​其它名称GABA A Receptor a2
  GABA A Receptor alpha 2
  GABA A receptor subunit alpha 2
  GABA
  GABA(A) receptor subunit alpha 2
  GABA(A) receptor subunit alpha-2
  GABR A2
  GABRA 2
  GABRA2
  GABRA2 protein
  Gamma aminobutyric acid (GABA) A receptor alpha 2
  Gamma aminobutyric acid A receptor alpha 2
  Gamma aminobutyric acid receptor alpha 2 subunit
  Gamma aminobutyric acid receptor subunit alpha 2
  Gamma-aminobutyric acid receptor subunit alpha-2
  GBRA2_HUMAN.

  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800
  FC:1μg/Test

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  Fig1: Sample:
  Cerebrum (Mouse) Lysate at 40 ug
  Primary: Anti-GABRA2  at 1/1000 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 48 kD
  Observed band size: 48 kD

   

  

  Fig2: Sample:
  Cerebral cortex(Rat) Lysate at 40 ug
  Primary: Anti-GABRA2 at 1/1000 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 48 kD
  Observed band size: 48 kD

   

  

  Fig3: Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
  Incubation: Anti-GABRA2 Polyclonal Antibody, Unconjugated( 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

   

  

  Fig4: Sample: Cerebrum (mouse) Lysate at 40 ug
  Primary: Anti- GABRA2 at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 48 kD
  Observed band size: 50 kD

   

  

  Fig5: Blank control(blue):RSC96 (fixed with 2% paraformaldehyde (10 min)).
  Primary Antibody:Rabbit Anti- GABRA2 antibody, Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
  Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
  Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

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