Anti-CRTC3 antibody [G8-A9]

Anti-CRTC3 antibody [G8-A9]

• 概述

• 产品描述Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates the expression of specific CREB-activated genes such as the steroidogenic gene, StAR. Potent coactivator of PPARGC1A and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR) .Tissue specificity: Predominantly expressed in B and T lymphocytes. Highest levels in lung. Also expressed in brain, colon, heart, kidney, ovary, and prostate. Weak expression in liver, pancreas, muscle, small intestine, spleen and stomach.
• 产品名称Anti-CRTC3 antibody [G8-A9]
• 分子量68kDa, additional band 43kDa
• 种属反应性Human,Monkey
• 验证应用WB,ICC,IHC-P
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Cytoplasm. Nucleus.
• 其它名称
  • CREB regulated transcription coactivator 3 antibody
  • CREB-regulated transcription coactivator 3 antibody
  • CRTC 3 antibody
  more

• 应用

  WB: 1:500-1:2,000
  ICC: 1:100-1:500
  IHC-P: 1:100-1:500

•  

  

  Fig1: Western blot analysis of CRTC3 on different cell lysates using anti-CRTC3 antibody at 1/1,000 dilution.
  Positive control:
  Lane 1: Hela
  Lane 2: Jurkat
  Lane 3: Cos7
  Lane 4: MCF-7

   

  

  Fig2: ICC staining CRTC3 (green) and actin filaments (red) in NTERA-2 cells. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig3: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-CRTC3 antibody. Counter stained with hematoxylin.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-CRTC3 antibody. Counter stained with hematoxylin.

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