Anti-ROR Gamma antibody
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概述
- 产品描述Possible nuclear receptor for hydroxycholesterols, the binding of which strongly promotes coactivators recruitment. Essential for thymopoiesis and the development of several secondary lymphoid tissues, including lymph nodes. Involved in lineage specification of uncommitted CD4(+) T helper cells into Th17 cells. Regulate the expression of several components of the circadian clock.
- 产品名称Anti-ROR Gamma antibody
- 分子量57 KDa
- 种属反应性Human,Mouse,Rat,Dog,Pig,Cow,Horse
- 验证应用WB,IHC-P,FC
- 抗体类型兔多抗
- 免疫原KLH conjugated synthetic peptide derived from human RORG 415-518/518
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
- 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 亚型IgG
- 纯化方式affinity purified by Protein A
- 亚细胞定位Nucleus (Probable).
- 其它名称RORG
NR1F3
Nuclear receptor ROR gamma
Nuclear receptor ROR-gamma
Nuclear receptor RZR gamma
Nuclear receptor RZR-gamma
Nuclear receptor subfamily 1 group F member 3
RAR related orphan receptor C
RAR related orphan receptor C, isoform a
RAR related orphan receptor gamma
Retinoic acid binding receptor gamma
Retinoid related orphan receptor gamma
Retinoid-related orphan receptor-gamma
Rorc
RORG_HUMAN
RZR GAMMA
RZRG
TOR.
more
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应用
WB:1:500-2000
IHC-P:1:400-800
FC:1ug/test
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Fig1: Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti-ROR Gamma at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57 kD
Observed band size: 57 kD
Fig2: Sample:
Muscle (Mouse) Lysate at 40 ug
Primary: Anti-ROR Gamm at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57 kD
Observed band size: 57 kD
Fig3: Blank control:Molt-4.
Primary Antibody (green line): Rabbit Anti-ROR Gamma antibody
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Fig4: Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (RORG) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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