Anti-Cytokeratin 7 antibody
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概述
- 产品描述Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).
- 产品名称Anti-Cytokeratin 7 antibody
- 分子量54 KDa
- 种属反应性Human,Mouse,Rat
- 验证应用WB,IHC-P
- 抗体类型兔多抗
- 免疫原KLH conjugated synthetic peptide derived from the middle of mouse CK7 251-350/469
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
- 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 亚型IgG
- 纯化方式affinity purified by Protein A
- 亚细胞定位Cytoplasm.
- 其它名称CK 7
CK-7
ck7
Cytokeratin 7
Cytokeratin-7
Cytokeratin7
D15Wsu77e
K2C7
K2C7_HUMAN
K7
Keratin 55k type ii cytoskeletal
Keratin 7
Keratin simple epithelial type 1 k7
Keratin type II cytoskeletal 7
Keratin type ii cytoskletal 7
Keratin, 55K type II cytoskeletal
Keratin, simple epithelial
Keratin, simple epithelial type I, K7
Keratin, type II cytoskeletal 7
Keratin-7
Keratin7
KRT 7
Krt2-7
KRT7
MGC11625
MGC129731
mgc3625
Sarcolectin
SCL
Type ii mesothelial keratin k7
Type-II keratin Kb7
more
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应用
WB:1:500-2000
IHC-P:1:400-800
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Fig1: Sample:
Large intestine (Mouse) Lysate at 40 ug
Large intestine(Rat) Lysate at 40 ug
Primary: Anti- Cytokeratin 7at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kD
Observed band size: 54 kD
Fig2: Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Cytokeratin 7 Polyclonal Antibody, Unconjugated1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Fig3: Sample: Large intestine (Mouse) Lysate at 30 ug
Primary: Anti- Cytokeratin 7at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilution
Predicted band size: 54 kD
Observed band size: 54 kD
Fig4: Sample:
Placenta (Mouse) Lysate at 30 ug
Lung (Mouse) Lysate at 30 ug
Primary: Anti-Cytokeratin 7t 1/300 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilution
Predicted band size: 54 kD
Observed band size: 54 kD
Fig5: Sample:
Embryo (Mouse) Lysate at 40 ug
Primary: Anti- Cytokeratin at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kD
Observed band size: 54 kD
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
