Anti-CARM1 antibody [B10-H2]

Anti-CARM1 antibody [B10-H2]

• 概述

• 产品描述CARM1 (coactivator-associated arginine methyltransferase 1), also known as protein arginine N-methyltransferase 4 (PRMT4), is a 585 amino acid nuclear and cytoplasmic protein belonging to the protein arginine N-methyltransferase family. As a protein arginine N-methyltransferase, CARM1 is capable of catalyzing the transfer of methyl groups from S-adenosylmethionine to the guanidino group nitrogen atoms of arginine residues in certain proteins involved in mRNA stability, DNA packaging and transcriptional regulation. The methyltransferase activity of CARM1 has been found to be negatively regulated through phosphorylation at a conserved serine residue. CARM1 acts as a positive regulator for multiple transcription factors and functions as a secondary coactivator through its association with p160 coactivators.
• 产品名称Anti-CARM1 antibody [B10-H2]
• 分子量65 kDa
• 种属反应性Human,Monkey,Rat,Mouse
• 验证应用WB,ICC,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Cytoplasm. Nucleus. Nucleoplasm.
• 其它名称
  • carm1 antibody
  • CARM1_HUMAN antibody
  • Coactivator associated arginine methyltransferase 1 antibody
  more

• 应用

  WB: 1:500-1:1,000
  ICC: 1:100-1:500
  IHC-P: 1:100-1:500
  FC: 1:100-1:200

•  

  

  Fig1: Western blot analysis of PRMT4 on different cell lysates using anti- PRMT4 antibody at 1/1,000 dilution.
  Positive control:
  Lane 1: MCF-7
  Lane 2: Hela
  Lane 3: NIH/3T3
  Lane 4: HL-60
  Lane 5: LNcap
  Lane 6: Jurkat
  Lane 7: PC-3
  Lane 8: Cos7
  Lane 9: PC-12

   

  

  Fig2: ICC staining PRMT4 (green) and actin filaments (red) in Hela cells. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig3: Immunohistochemical analysis of paraffin-embedded breast cancer tissue using anti-PRMT4 antibody. Counter stained with hematoxylin.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded ovarian cancer tissue using anti-PRMT4 antibody. Counter stained with hematoxylin.

   

  

  Fig5: Flow cytometric analysis of Lovo cells with PRMT4 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).

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