Anti-AF10 antibody

Anti-AF10 antibody

• 概述

• 产品描述Probably involved in transcriptional regulation. In vitro or as fusion protein with MLL has transactivation activity. Binds to cruciform DNA.
• 产品名称Anti-AF10 antibody
• 分子量109 KDa
• 种属反应性Human,Mouse,Rat,Dog,Pig,Cow,Horse,Rabbit,Sheep
• 验证应用WB,FC
• 抗体类型兔多抗
• 免疫原KLH conjugated synthetic peptide derived from human AF10/MLLT10 151-250/1027
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• 亚型IgG
• 纯化方式affinity purified by Protein A
• 亚细胞定位Nucleus.
• 其它名称AF 10
  AF10
  AF10_HUMAN
  ALL 1 fused gene from chromosome 10
  ALL1 fused gene from chromosome 10
  ALL1-fused gene from chromosome 10 protein
  MLLT 10
  MLLT10
  Myeloid/lymphoid or mixed lineage leukemia (trithorax homolog Drosophila) translocated to 10
  Myeloid/lymphoid or mixed lineage leukemia translocated to 10
  Protein AF 10
  Protein AF-10
  Protein AF10
  Type I AF10 protein.
  more

• 应用

  WB:1:500-2000
  FC:1μg /Test

•  

  

  Fig1: Blank control: U-2OS(blue)
  Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μl in 100 μL1X PBS containing 0.5% BSA(green).

   

  

  Fig2: Sample: Testis (Rat) Lysate at 40 ug
  Primary: Anti-AF10  at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 109 kD
  Observed band size: 109 kD

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