Anti-c-Jun antibody
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概述
- 产品描述Genes belonging to the Jun and Fos oncogene families encode nuclear proteins that are associated with a number of transcriptional complexes. The c-Jun protein is a major component of the transcription factor AP-1, originally shown to mediate phorbol ester tumor promoter (TPA)-induced expression of responsive genes through the TPA-response element (TRE). The Jun proteins form homo- and heterodimers which bind the TRE, while Fos proteins are active only as heterodimers with any of the Jun proteins. Fos/Jun heterodimers have a much higher affinity for the TRE than Jun homodimers. Ha-Ras augments c-Jun activity and stimulates phosphorylation of its activation domain. An inhibitor of Fos/Jun function, termed IP-1, associates with Fos and Jun and is inactivated upon phosphorylation induced by the cAMP-dependent protein kinase A (PKA).
- 产品名称Anti-c-Jun antibody
- 分子量39kDa
- 种属反应性Human, Mouse, Rat
- 验证应用WB, IP, IF, IHC-P
- 抗体类型兔多抗
- 免疫原peptide
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃
- 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol.
- 亚型IgG
- 纯化方式Immunogen affinity purified
- 亚细胞定位Nucleus
- 其它名称
- Activator protein 1 antibody
- AP 1 antibody
- AP1 antibody
more
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应用
WB: 1:100-1:1,000
IP: 1-2 μg per 100-500 μg of total protein (1 ml of cell lysate)
IF: 1:50-1:500
IHC-P: 1:50-1:500
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Fig1: Western blot analysis of c-Jun phosphorylation in nontransfected (A,D), untreated mouse c-Jun transfected (B,E) and lambda protein phosphatase treated human c-Jun transfected(C,F) 293T whole cell lysates.
Fig2: Immunofluorescence staining of formalin-fixed HeLa cells showing nuclear localization. (A). Immunoperoxidase staining of formalin-fixed, paraffinembedded normal human prostate tissue showing nuclear localization (B).
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