Anti-AMACR/P504S antibody [6F5]

Anti-AMACR/P504S antibody [6F5]

• 概述

• 产品描述AMACR, is an enzyme that responsible for converting (2R)-methylacyl-CoA esters to their (2S)-methylacyl-CoA epimers and known substrates, including coenzyme A esters of pristanic acid (mostly derived from phytanic acid, a 3-methyl branched-chain fatty acid that is abundant in the diet) and bile acids derived from cholesterol. Both decreased and increased levels of the enzyme in humans are linked with diseases. Reduction of the protein level or activity results in the accumulation of (2R)-methyl fatty acids such as bile acids which causes neurological symptoms. Increased levels of AMACR protein concentration and activity are associated with prostate cancer, and the enzyme is used widely as a biomarker (known in cancer literature as P504S) in biopsy tissues. Increased levels of AMACR are also associated with some breast, colon, and other cancers, but it is unclear exactly what the role of AMACR is in these cancers. This AMACR antibody is intended for qualified laboratories to qualitatively identify by light microscopy the presence of associated antigens in sections of formalinfixed, paraffin-embedded tissue sections using IHC test methods.
• 产品名称Anti-AMACR/P504S antibody [6F5]
• 分子量42 kDa
• 种属反应性Human
• 验证应用WB,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant full length protein of human AMACR.
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein G purified.
• 亚细胞定位Mitochondrion. Peroxisome.
• 其它名称
  • 2 arylpropionyl CoA epimerase antibody
  • 2 methylacyl CoA racemase antibody
  • 2-methylacyl-CoA racemase antibody
  more

• 应用

  WB: 1:500-1:2,000
  IHC-P: 1:50-1:200
  FC: 1:50-1:100

•  

  

  Fig1: Western blot analysis of AMACR/P504S on Siha cell and human liver tissue lysates using anti-AMACR/P504S antibody at 1/1,000 dilution.

   

  

  Fig2: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti- AMACR/P504S antibody. Counter stained with hematoxylin.

   

  

  Fig3: Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti- AMACR/P504S antibody. Counter stained with hematoxylin.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti- AMACR/P504S antibody. Counter stained with hematoxylin.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti- AMACR/P504S antibody. Counter stained with hematoxylin.

   

  

  Fig6: Flow cytometric analysis of A549 cells with AMACR/P504S antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！