Anti-SREBP1 antibody
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概述
- 产品描述This gene encodes a transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a decamer flanking the low density lipoprotein receptor gene and some genes involved in sterol biosynthesis. The protein is synthesized as a precursor that is attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription by binding to the SRE1. Sterols inhibit the cleavage of the precursor, and the mature nuclear form is rapidly catabolized, thereby reducing transcription. The protein is a member of the basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factor family. This gene is located within the Smith-Magenis syndrome region on chromosome 17.
- 产品名称Anti-SREBP1 antibody
- 分子量54/126 KDa
- 种属反应性Human,Mouse,Rat,Chicken
- 验证应用WB,IHC-P
- 抗体类型兔多抗
- 免疫原KLH conjugated synthetic peptide derived from human SREBP-1 301-450/1147
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
- 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 亚型IgG
- 纯化方式affinity purified by Protein A
- 亚细胞定位Endoplasmic reticulum membrane. Nucleus.
- 其它名称Sterol regulatory element binding protein 1
ADD 1
BHLHD1
D630008H06
SREBF 1
SREBP-1
SREBF1
SREBP 1
SREBP1
SREBP 1c
Sterol regulatory element binding protein 1
Sterol Regulatory Element Binding Transcription Factor 1 / Protein 1
Sterol regulatory element binding transcription factor 1
SRBP1_HUMAN
Sterol regulatory element-binding protein 1
SREBP-1
Class D basic helix-loop-helix protein 1
bHLHd1
Sterol regulatory element-binding transcription factor 1
Processed sterol regulatory element-binding protein 1.
more
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应用
WB:1:500-2000
IHC-P:1:400-800
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Fig1: Paraformaldehyde-fixed, paraffin embedded (rat adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (SREBP1) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Fig2: Sample:
Lung(Mouse) Lysate at 30 ug
Ovary (Mouse) Lysate at 30 ug
Skin (Mouse) Lysate at 30 ug
Primary: Anti- SREBP1 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54/126 kD
Observed band size: 50 kD
Fig3: Sample: Muscle(Mouse) Lysate at 30 ug
Primary: Anti- SREBP1at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54/126 kD
Observed band size: 50 kD
Fig4: Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (SREBP-1) Polyclonal Antibody, Unconjugatedat 1:200 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Fig5: Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (SREBP-1) Polyclonal Antibody, Unconjugatedat 1:200 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Fig6: Sample:
Liver(Mouse) Cell Lysate at 40 ug
Liver(Rat) Cell Lysate at 40 ug
Primary: Anti-SREBP1 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54/126 kD
Observed band size: 54 kD
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