Anti-CAPRIN2 antibody

Anti-CAPRIN2 antibody

• 概述

• 产品描述May regulate the transport and translation of mRNAs, modulating for instance the expression of proteins involved in synaptic plasticity in neurons. Involved in regulation of growth as erythroblasts shift from a highly proliferative state towards their terminal phase of differentiation. May be involved in apoptosis.

• 产品名称Anti-CAPRIN2 antibody

• 分子量126 KDa

• 种属反应性Human,Mouse,Rat,Chicken,Pig,Horse,Sheep

• 验证应用WB,IHC-P

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human CAPRIN2 101-200/1127

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Cytoplasm; Mitochondrion. Colocalizes with aggregated mitochondria and Mitochondrion. Cytoplasm. Expressed throughout the cytoplasm.

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• ​其它名称C1q domain-containing protein 1
  C1QDC1
  CAPR2_HUMAN
  Caprin family member 2
  Caprin-2
  caprin2
  Cytoplasmic activation/proliferation-associated protein 2
  EEG 1
  EEG1
  Gastric cancer multidrug resistance-associated protein
  KIAA1873
  Protein EEG-1
  RNA granule protein 140
  RNG140.

  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800

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  Fig1: Sample:
  U87MG(Human) Cell Lysate at 30 ug
  U2OS(Human) Cell Lysate at 30 ug
  Primary: Anti- CAPRIN2  at 1/1000 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 126 kD
  Observed band size: 126 kD

   

  

  Fig2: Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (CAPRIN2) Polyclonal Antibody, Unconjugated  at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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