Anti-GAPDHS antibody
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概述
- 产品描述May play an important role in regulating the switch between different pathways for energy production during spermiogenesis and in the spermatozoon. Required for sperm motility and male fertility.
- 产品名称Anti-GAPDHS antibody
- 分子量45 KDa
- 种属反应性Human,Mouse,Rat,Dog,Cow,Horse,Sheep
- 验证应用WB,IHC-P
- 抗体类型兔多抗
- 免疫原KLH conjugated synthetic peptide derived from human GAPDHS 251-350/408
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
- 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 亚型IgG
- 纯化方式affinity purified by Protein A
- 亚细胞定位Cytoplasm.
- 其它名称GAPDH2
GAPD2
GAPDH 2
GAPDH 2
GAPDHS
GAPDS
Glyceraldehyde 3 phosphate dehydrogenase spermatogenic
Glyceraldehyde 3 phosphate dehydrogenase, testis specific
HSD 35
HSD35
Spermatogenic cell specific glyceraldehyde 3 phosphate dehydrogenase 2
Spermatogenic glyceraldehyde 3 phosphate dehydrogenase
G3PT_HUMAN
HSD-35.
more
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应用
WB:1:500-2000
IHC-P:1:400-800
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Fig1: Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GAPDHS Polyclonal Antibody, Unconjugated 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Fig2: Sample:
Testis (Mouse) Lysate at 40 ug
Primary: Anti-GAPDHS at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kD
Observed band size: 47 kD
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
