Anti-USP21 antibody
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概述
- 产品描述The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. Through the use of a wide range of enzymes that can add or remove ubiquitin, the Ub pathway controls many intracellular processes such as signal transduction, transcriptional activation and cell cycle progression. USP21 (ubiquitin specific peptidase 21), also known as USP16 or USP23, is a 565 amino acid protein that belongs to the C19 peptidase family of ubiquitin carboxy-terminal hydrolases. Capable of removing ubiquitin from ubiquitinated proteins, USP21 plays a role in signal transduction and can also remove NEDD8 from NEDD8-conjugated proteins, possibly functioning to influence NEDD8-mediated protein proteolysis. Multiple isoforms of USP21 exist due to alternative splicing events.
- 产品名称Anti-USP21 antibody
- 分子量63 kDa
- 种属反应性Human,Rat,Mouse
- 验证应用WB,ICC,IHC-P,FC
- 抗体类型兔多抗
- 免疫原Peptide
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Peptide affinity purified
- 亚细胞定位Cytoplasm. Nucleus.
- 其它名称Deubiquitinating enzyme 21 antibody
deubiquitinating enzyme 23 antibody
EC 3.1.2.15 antibody
ESTM28 antibody
MGC3394 antibody
NEDD8-specific protease antibody
Ubiquitin carboxyl terminal hydrolase 21 antibody
ubiquitin carboxyl terminal hydrolase 23 antibody
Ubiquitin carboxyl-terminal hydrolase 21 antibody
ubiquitin specific peptidase 21 antibody
Ubiquitin specific processing protease 21 antibody
ubiquitin specific protease 16 antibody
ubiquitin specific protease 21 antibody
ubiquitin specific protease 23 antibody
Ubiquitin thioesterase 21 antibody
Ubiquitin thiolesterase 21 antibody
ubiquitin thiolesterase 23 antibody
ubiquitin-specific protease 23, formerly antibody
Ubiquitin-specific-processing protease 21 antibody
UBP21_HUMAN antibody
USP16 antibodyv Usp21 antibody
USP23 antibody
USP23, formerly antibody
W53272 antibody
more
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应用
WB: 1:500
ICC: 1:500-1:2,000
IHC-P: 1:50-1:200
FC: 1:50-1:100
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Fig1: Western blot analysis of USP21 on mouse thymus tissue lysate using anti-USP21 antibody at 1/200 dilution.
Fig2: ICC staining USP21 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining USP21 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-USP21 antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-USP21 antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-USP21 antibody. Counter stained with hematoxylin.
Fig7: Flow cytometric analysis of SH-SY-5Y cells with USP21 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secon
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