Anti-PINCH antibody [B9-G7]

Anti-PINCH antibody [B9-G7]

• 概述

• 产品描述Pinch, also designated particularly interesting new Cys-His protein or NY-REN-48, is a focal adhesion protein that is a component of the ILK-PINCH complex. This complex is a major part of the growth factor and integrin signaling pathway. Pinch is involved in cell differentiation, proliferation and survival by acting as an effector of integrin and growth factor signaling. It is a cytoplasmic protein expressed in most tissues and consists of five LIM domains, a nuclear localization signal and a nuclear export signal. The PINCH-1/ILK complex is regulated by a PINCH-1 related protein PINCH-2, which also forms a complex with ILK.
• 产品名称Anti-PINCH antibody [B9-G7]
• 分子量37 kDa
• 种属反应性Human
• 验证应用WB,ICC,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Cell junction.
• 其它名称
  • 2310016J22Rik antibody
  • 4921524A02Rik antibody
  • AI507642 antibody
  more

• 应用

  WB: 1:500-1:1,000
  ICC: 1:100-1:500
  FC: 1:100-1:200

•  

  

  Fig1: Western blot analysis of PINCH on human PINCH recombinant protein using anti-PINCH antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of PINCH on different cell lysates using anti-PINCH antibody at 1/1,000 dilution.
  Positive control:
  Lane 1: A549
  Lane 2: Jurkat
  Lane 3: Hela

   

  

  Fig3: ICC staining PINCH (green) and actin filaments (red) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: Flow cytometric analysis of Hela cells with PINCH antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red).

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！