Anti-5HT4 Receptor antibody
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概述
- 产品描述This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that stimulate adenylate cyclase.
- 产品名称Anti-5HT4 Receptor antibody
- 分子量43 KDa
- 种属反应性Human,Mouse,Rat,Chicken,Cow
- 验证应用WB,IHC-P,ICC/IF
- 抗体类型兔多抗
- 免疫原KLH conjugated synthetic peptide derived from human 5-HTR4 21-120/388
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
- 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 亚型IgG
- 纯化方式affinity purified by Protein A
- 亚细胞定位Cell membrane; Multi-pass membrane protein. Endosome. Note=Interaction with SNX27 mediates recruitment to early endosomes, while interaction with SLC9A3R1 and EZR might target the protein to specialized subcellular regions, such as microvilli.
- 其它名称5-HTR4 (5-hydroxytryptamine (serotonin) receptor 4a)
Htr4a
5-HT4
5-HT4A
5HT4
Htr45 HT 4
5 HT4
5 HT4R
5 hydroxytryptamine 4 receptor
5 hydroxytryptamine receptor 4
5 hydroxytryptamine serotonin receptor 4
5 hydroxytryptamine4 receptor
5HT4
5HT4R
Cardiac 5 HT4 receptor
HTR 4
HTR4
Serotonin 5 HT4 receptor
Serotonin receptor 4
5HT4R_HUMAN.
more
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应用
WB:1:500-2000
IHC-P:1:400-800
IF:1:200-800
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Fig1: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (5HT4 Receptor) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Fig2: Sample:
Bone (Mouse) Lysate at 40 ug
Primary: Anti-5HT4 Receptor (bs- 2127R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kD
Observed band size: 43 kD
Fig3: Sample:
Large intestine (Mouse) Lysate at 40 ug
Primary: Anti-5HT4 Receptor at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kD
Observed band size: 43 kD
Fig4: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (5HT4 Receptor) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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