Anti-DYKDDDDK Tag(FLAG) antibody [A2-A4]

Anti-DYKDDDDK Tag(FLAG) antibody [A2-A4]

• 概述

• 产品描述The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. Due to its small size, it is unlikely to affect the tagged protein's biochemical properties. This antibody recognizes the DYKDDDDK peptide fused to either the amino- or carboxy-terminus of targeted proteins.
• 产品名称Anti-DYKDDDDK Tag(FLAG) antibody [A2-A4]
• 验证应用WB, IP, ELISA
• 抗体类型小鼠单抗
• 免疫原Synthetic peptide immune sequence is N-DYKDDDDK-C.
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein G purified.
• 其它名称
  • DDDDK epitope tag antibody
  • DDDK antibody
  • ddk antibody
  more

• 应用

  WB: 1:5,000-1:10,000
  IP: 1:10-1:50

•  

  

  Fig1: Western blot analysis of DYKDDDDK Tag(FLAG) on DYKDDDDK Tag recombinant protein (65kDa). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (M1403-2, 1/5,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.

   

  

  Fig2: Western blot analysis of DYKDDDDK Tag(FLAG) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (M1403-2, 1/5,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
  Positive control:
  Lane 1: C-terminal FLAG-tagged recombinant protein
  Lane 2: N-terminal FLAG-tagged recombinant protein

   

  

  Fig3: Immunoprecipitation analysis of different lysates using anti-FLAG antibody. Lane 1: Non-transfected 293T Lane 2: Transfected 293T Lane 3: Agarose beads complexes Lane 4: Negative control (Normal mouse IgG)

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