Anti-DGAT1 antibody
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概述
- 产品描述Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. In contrast to DGAT2 it is not essential for survival. May be involved in VLDL (very low density lipoprotein) assembly. In liver, plays a role in esterifying exogenous fatty acids to glycerol. Functions as the major acyl-CoA retinol acyltransferase (ARAT) in the skin, where it acts to maintain retinoid homeostasis and prevent retinoid toxicity leading to skin and hair disorders.
- 产品名称Anti-DGAT1 antibody
- 分子量55 KDa
- 种属反应性Human,Mouse,Rat,Dog,Pig,Horse
- 验证应用WB,IHC-P
- 抗体类型兔多抗
- 免疫原KLH conjugated synthetic peptide derived from human DGAT1 251-350/488
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
- 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 亚型IgG
- 纯化方式affinity purified by Protein A
- 亚细胞定位Endoplasmic reticulum membrane; Multi-pass membrane protein.
- 其它名称ACAT related gene product 1
ACAT-related gene product 1
Acyl coenzyme A:cholesterol acyltransferase related gene 1
Acyl-CoA retinol O-fatty-acyltransferase
Acyl-CoA:diacylglycerol acyltransferase
ARAT
ARGP1
C75990
D15Ertd23e
Dgat
DGAT1
DGAT1_HUMAN
Diacylglycerol O acyltransferase 1
Diacylglycerol O-acyltransferase 1
Diglyceride acyltransferase
EC 2.3.1.20
hCG_24006
MGC139064
Retinol O fatty acyltransferase.
more
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应用
WB:1:500-2000
IHC-P:1:400-800
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Fig1: Sample: Hela (human)cell Lysate at 40 ug
Primary: Anti- DGAT1at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55kD
Observed band size: 55 kD
Fig2: Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (DGAT1) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Fig3: Sample:small intesitne (mouse) Lysate at 40 ug
Primary: Anti-DGAT1at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 67kD
Observed band size: 67 kD
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
