Anti-C1s antibody
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概述
- 产品描述C1s B chain is a serine protease that combines with C1q and C1r to form C1, the first component of the classical pathway of the complement system. C1r activates C1s so that it can, in turn, activate C2 and C4.
- 产品名称Anti-C1s antibody
- 分子量47/75 KDa
- 种属反应性Human,Mouse,Rat,Horse
- 验证应用WB,IHC-P
- 抗体类型兔多抗
- 免疫原KLH conjugated synthetic peptide derived from human C1s 251-350/688
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
- 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 亚型IgG
- 纯化方式affinity purified by Protein A
- 其它名称Basic proline rich peptide IB 1
C1 esterase
C1S
C1S_HUMAN
Complement C1s subcomponent
Complement C1s subcomponent heavy chain
Complement C1s subcomponent light chain
Complement component 1 s subcomponent
Complement component 1 subcomponent s
FLJ44757.
more
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应用
WB:1:500-2000
IHC-P:1:400-800
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Fig1: Sample:
Pancreas (Mouse) Lysate at 40 ug
Primary: Anti-C1s at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47/75 kD
Observed band size: 47 kD
Fig2: Sample: Heart (Mouse) Lysate at 40 ug
Primary: Anti-C1sat 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47/75 kD
Observed band size: 55 kD
Fig3: Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-C1S Polyclonal Antibody, Unconjugated1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
