Anti-Carcino Embryonic Antigen CEA antibody
-
概述
- 产品描述The CD66 (carcinoembryonic antigen, CEA, biliary glycoprotein I, BGP-1, CEACAM) immunoglobulin superfamily of genes encode cell adhesion proteins, which are expressed at higher levels in tumorous tissues than in normal tissues. The human CD66 gene family is a diverse set of glycoproteins of epithelial and hematopoietic lineage that comprises 29 genes, which map to chromosome position 19q13.1-q13.2. CD66A, CD66B, CD66C, CD66D, CD66E and CD66F are the best characterized CD66 antigens, and CD66A-D expression upregulates on the surface of granulocytes upon stimulation. Certain CD66 family members mediate homotypic and heterotypic intercellular adhesion events. CD66E, also known as CEA, is a well known tumor marker and a heavily glycosylated GPI-linked cell surface molecule.
- 产品名称Anti-Carcino Embryonic Antigen CEA antibody
- 分子量150 kDa (predicted molecular weight: 76 kDa)
- 种属反应性Human
- 验证应用WB,ICC,IHC-P,FC
- 抗体类型兔多抗
- 免疫原Synthetic peptide corresponding to a region of human Carcino Embryonic Antigen CEA.
- 偶联Non-conjugated
-
性能
- 形态Liquid
- 浓度2 mg/ml.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 25% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Peptide affinity purified.
- 亚细胞定位Membrane.
- 数据链接SwissProt: P06731 Human
- 其它名称Carcinoembryonic antigen antibody
Carcinoembryonic antigen-related cell adhesion molecule 5 antibody
CD66e antibody
CEA antibody
Ceacam5 antibody
CEAM5_HUMAN antibody
DKFZp781M2392 antibody
Meconium antigen 100 antibody
OTTHUMP00000199032 antibody
OTTHUMP00000199033 antibody
OTTHUMP00000199034 antibody
more
-
应用
WB:1:1,000-1:2,000
ICC:1:50-1:200
IHC-P:1:50-1:200
FC:1:50-1:100
-
Fig1: Western blot analysis of Carcino Embryonic Antigen CEA on MCF-7 lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Fig2: ICC staining Carcino Embryonic Antigen CEA in SK-Br-3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Carcino Embryonic Antigen CEA polyclonal antibody at a dilution of 1:200 for at least 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
Fig3: ICC staining Carcino Embryonic Antigen CEA in SW620 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Carcino Embryonic Antigen CEA polyclonal antibody at a dilution of 1:200 for at least 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
Fig4: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Carcino Embryonic Antigen CEA antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/100 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Carcino Embryonic Antigen CEA antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/100 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
Fig6: Flow cytometric analysis of Carcino Embryonic Antigen CEA was done on HepG2 cells. The cells were fixed, permeabilized and stained with Carcino Embryonic Antigen CEA antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). After incubation of the primary antibody on room temperature for an hour, the cells was stained with a Alexa Fluor™ 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
