Anti-AMPK gamma1 antibody [5A1]

Anti-AMPK gamma1 antibody [5A1]

• 概述

• 产品描述AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive.
• 产品名称Anti-AMPK gamma1 antibody [5A1]
• 分子量36 KDa
• 种属反应性Human
• 验证应用WB
• 抗体类型小鼠单抗
• 免疫原Recombinant human AMPK gamma1 Protein.
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
• 存储缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• 亚型IgG1
• 纯化方式affinity purified by Protein A
• 亚细胞定位Cytosol; Nucleus.
• 其它名称
  • PRKAG 1
  • PRKAG1
  • 5 -AMP-activated protein kinase gamma-1 subunit
  more

• 应用

  WB:1:500-2000

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  Fig1: Sample:
  U87MG(Human) Cell Lysate at 30 ug
  Primary: Anti- AMPK gamma1 (EM2001-06) at 1/1000 dilution
  Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
  Predicted band size: 36 kD
  Observed band size: 36 kD

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！