Anti-Ikk alpha antibody

Anti-Ikk alpha antibody

• 概述

• 产品描述Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. It acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis.

• 产品名称Anti-Ikk alpha antibody

• 分子量85 kDa

• 种属反应性Human,Mouse,Rat

• 验证应用WB,ICC,IHC-P,FC

• 抗体类型兔多抗

• 免疫原peptide

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

• 亚型IgG

• 纯化方式Peptide affinity purified

• 亚细胞定位Cytoplasm, nucleus.

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• ​其它名称chuk antibody
  CHUK1 antibody
  Conserved Helix Loop Helix Ubiquitous Kinase antibody
  Conserved helix loop ubiquitous kinase antibody
  Conserved helix-loop-helix ubiquitous kinase antibody
  I Kappa B Kinase 1 antibody
  I Kappa B Kinase Alpha antibody
  I-kappa-B kinase 1 antibody
  I-kappa-B kinase alpha antibody
  IkappaB kinase antibody
  IkB kinase alpha subunit antibody
  IkBKA antibody
  IKK 1 antibody
  IKK A antibody
  IKK a kinase antibody
  IKK-A antibody
  IKK-alpha antibody
  IKK1 antibody
  IKKA antibody
  IKKA_HUMAN antibody
  Inhibitor Of Kappa Light Polypeptide Gene Enhancer In B Cells antibody
  Inhibitor Of Nuclear Factor Kappa B Kinase Alpha Subunit antibody
  Inhibitor of nuclear factor kappa-B kinase subunit alpha antibody
  NFKBIKA antibody
  Nuclear Factor Kappa B Inhibitor Kinase Alpha antibody
  Nuclear factor NF kappa B inhibitor kinase alpha antibody
  Nuclear factor NF-kappa-B inhibitor kinase alpha antibody
  Nuclear factor NFkappaB inhibitor kinase alpha antibody
  Nuclear Factor Of Kappa Light Chain Gene Enhancer In B Cells Inhibitor antibody
  TCF-16 antibody
  TCF16 antibody
  Transcription factor 16 antibody

  more

• 应用

  WB: 1:500
  ICC: 1:200
  IHC-P: 1:200
  FC: 1:100-1:200

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  Fig1: Western blot analysis of IKK alpha on different cell lysates using anti-IKK alpha antibody at 1/500 dilution.
  Positive control:
  Lane 1: Hela
  Lane 2: HepG2
  Lane 3: Raji
  Lane 4: NIH/3T3
  Lane 5: PC12
  Lane 6: Jurkat

   

  

  Fig2: ICC staining IKK alpha in N2A cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig3: Immunohistochemical analysis of paraffin-embedded human mammary gland tissue using anti-IKK alpha antibody. Counter stained with hematoxylin.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-IKK alpha antibody. Counter stained with hematoxylin.

   

  

  Fig5: Flow cytometric analysis of Hela cells with IKK alpha antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.

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