Anti-GRP94 antibody [F1-D10-G3]

Anti-GRP94 antibody [F1-D10-G3]

• 概述

• 产品描述The human HSP 90 family includes 17 genes that fall into four classes: HSP90AA, HSP90AB, HSP90B and TRAP. HSP 90 family members guide the normal folding, intracellular disposition and Proteolytic turnover of many key regulators of cell growth, differentiation and survival. HSP 90α, also designated HSP90A, HSP 86 and LPS-associated protein 2 (LAP2), is a cytosolic enhancer of inducible nitric-oxide synthase (iNOS), with chaperone activity that is important for the transcriptional activity of p53. HSP 90β, also designated HSP90B, HSP 84 and HSPC2, is a cytosolic protein that participates in signaling pathways with PKC e to protect cells from external damage, particularly in heat shock-mediated events. GRP 94, also known as tumor rejection antigen 1 (TRA1), ECGP and GP96, localizes to the ER, is highly expressed in BGC-823 human gastric carcinoma cells and is upregulated in human endothelial cells in response to hypoxia by HIF-1.
• 产品名称Anti-GRP94 antibody [F1-D10-G3]
• 分子量94 kDa
• 种属反应性Human,Mouse
• 验证应用WB,IHC-P,ICC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgM
• 纯化方式Protein A purified.
• 亚细胞定位Endoplasmic reticulum lumen, melanosome.
• 其它名称
  • 94 kDa glucose regulated protein antibody
  • 94 kDa glucose-regulated protein antibody
  • ECGP antibody
  more

• 应用

  WB: 1:1,000-1:10,000
  ICC: 1:50
  IHC-P: 1:50-1:200

•  

  

  Fig1: Western blot analysis of GRP94 on different cell lysates using anti-GRP94 antibody at 1/5,000 dilution.
  Positive control:
  Lane 1: NIH-3T3
  Lane 2: Hela
  Lane 3: HepG2

   

  

  Fig2: ICC staining GRP94 (green) in NIH-3T3 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig3: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-GRP94 antibody. Counter stained with hematoxylin.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-GRP94 antibody. Counter stained with hematoxylin.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-GRP94 antibody. Counter stained with hematoxylin.

   

  

  Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-GRP94 antibody. Counter stained with hematoxylin.

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