Anti-Sema3A antibody
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概述
- 产品描述Plays a role in growth cones guidance. May function to pattern sensory projections by selectively repelling axons that normally terminate dorsally. Involved in the development of the olfactory system and in neuronal control of puberty.
- 产品名称Anti-Sema3A antibody
- 分子量86 KDa
- 种属反应性Human,Mouse,Rat
- 验证应用WB,IHC-P
- 抗体类型兔多抗
- 免疫原KLH conjugated synthetic peptide derived from mouse Sema3A 22-120/772
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
- 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 亚型IgG
- 纯化方式affinity purified by Protein A
- 亚细胞定位Secreted.
- 其它名称Semaphorin 3A
Semaphorin-3A
Semaphorin-3A precursor
Semaphorin3A
SemD
Sema 3A
SEM3A_MOUSE
Coll 1
Coll1
Hsema I
Hsema III
sema domain immunoglobulin domain (Ig) short basic domain secreted (semaphorin) 3A
Sema III
SEMA1
SEMA1-PEN
SEMA3A
SEMAD
SEMAIII
SEMAL
Semaphorin III
Semaphorin like growth cone guidance homolog of chick collapsin
Semaphorin III
Semaphorin-D
Sema D.
more
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应用
WB:1:500-2000
IHC-P:1:400-800
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Fig1: Sample:
Lung (Mouse) Lysate at 40 ug
Primary: Anti-Sema3A at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 86 kD
Observed band size: 86 kD
Fig2: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (Sema3A) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
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