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别名: | OX40L,TNFSF4,CD252,Glycoprotein Gp34,TXGP1,CD134 ligand,CD134L | ||
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储存条件: | -20℃ |
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储存条件: | -20℃ |
Biotinylated Human OX40 Ligand / TNFSF4 Protein, His,Avitag™ (active trimer) (MALS verified)
OX40L,TNFSF4,CD252,Glycoprotein Gp34,TXGP1,CD134 ligand,CD134L
Biotinylated Human OX40 Ligand, His,Avitag is expressed from human 293 cells. It contains AA Gln 51 - Leu 183 .
Predicted N-terminus: His
This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 51.1 kDa. The protein migrates as 65-80 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Passed as determined by the HABA assay / binding ELISA.
Less than 1.0 EU per μg by the LAL method.
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Biotinylated Human OX40 Ligand, His,Avitag on SDS-PAGE under non-reducing (NR) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
The purity of Biotinylated Human OX40 Ligand, His,Avitag is more than 90% and the molecular weight of this protein is around 57-78 kDa verified by SEC-MALS.
Report
Immobilized Human OX40, Fc Tag at 1 μg/mL (100 μL/well) can bind Biotinylated Human OX40 Ligand, His,Avitag with a linear range of 0.5-8 ng/mL (QC tested).