Biotinylated Cynomolgus IL-2 R beta&IL-2 R alpha&IL-2 R gamma Protein, Fc,Avitag™&Fc,Avitag™ (MALS verified)

Biotinylated Cynomolgus IL-2 R beta&IL-2 R alpha&IL-2 R gamma Protein, Fc,Avitag™&Fc,Avitag™ (MALS verified)

分分子别名（Synonym）

IL-2 R beta & IL-2 R alpha & IL-2 R gamma

表达区间及表达系统（Source）

Biotinylated Cynomolgus IL-2RB&IL-2RA&IL-2RG, Fc,Avitag&Fc,Avitag is expressed from human 293 cells . It contains AA Ala 27 - Thr 240 (IL-2RB) & Glu 22 - Glu 238 (IL-2RA) & Leu 23 - Pro 255 (IL-2RG) .

Predicted N-terminus: Ala 27 (IL-2RB) & Glu 22 (IL-2RA)

蛋白结构（Molecular Characterization）

Biotinylated Cynomolgus IL-2RB&IL-2RA&IL-2RG, Fc,Avitag&Fc,Avitag is produced by co-expression of IL-2RB and IL-2RA and IL-2RG, has a calculated MW of 53.0 kDa (IL-2RB) and 80.0 kDa (IL-2RA&IL-2RG). Subunit IL-2RB is fused with a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™) and subunit IL-2RA&IL-2RG is fused with a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™). The reducing (R) protein migrates as 65-70 kDa and 115-130 kDa respectively due to glycosylation.

标记（Biotinylation）

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度（Biotin:Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;

2. -70^°C for 3 months under sterile conditions after reconstitution.

电泳（SDS-PAGE）

Biotinylated Cynomolgus IL-2RB&IL-2RA&IL-2RG, Fc,Avitag&Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

The purity of Biotinylated Cynomolgus IL-2RB&IL-2RA&IL-2RG, Fc,Avitag&Fc,Avitag  is more than 85% and the molecular weight of this protein is around 200-240 kDa verified by SEC-MALS.

Report

活性（Bioactivity）-ELISA

Immobilized Human IL-2, His Tag  at 5 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus IL-2RB&IL-2RA&IL-2RG, Fc,Avitag&Fc,Avitag  with a linear range of 8-125 ng/mL (QC tested).

Protocol

活性（Bioactivity）-BLI

Loaded Biotinylated Cynomolgus IL-2RB&IL-2RA&IL-2RG, Fc,Avitag&Fc,Avitag  on SA Biosensor, can bind Human IL-2, His Tag with an affinity constant of 0.755 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

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