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| 别名: | CSF1R,C-FMS,CD115,CSFR,FIM2,FMS,M-CSFR | ||
|---|---|---|---|
| 储存条件: | -20℃ | ||
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| 货号 | 规格 | 可用库存 | 销售价(RMB) | 您的折扣价(RMB) | 购买数量 |
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| 熔点: | |
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| 密度: | |
| 储存条件: | -20℃ |
Human M-CSF R / CSF1R / CD115 Protein, His Tag (HPLC verified)
CSF1R,C-FMS,CD115,CSFR,FIM2,FMS,M-CSFR
Human M-CSF R, His Tag is expressed from human 293 cells . It contains AA Ile 20 - Glu 512 .
Predicted N-terminus: Ile 20
Request for sequence
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 55.3 kDa. The protein migrates as 80-95 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>96% as determined by SDS-PAGE.
>90% as determined by SEC-HPLC.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Human M-CSF R, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 96%.
The purity of Human M-CSF R, His Tag was greater than 90% as determined by SEC-HPLC.
Immobilized Human M-CSF R, His Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Human M-CSF, His,Avitagwith a linear range of 2-31 ng/mL (QC tested).
Protocol
Biotinylated Human M-CSF, His,Avitag immobilized on SA Chip can bind Human M-CSF R, His Tag with an affinity constant of 0.309 μM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
Loaded Biotinylated Human M-CSF, His,Avitag on SA Biosensor, can bind Human M-CSF R, His Tag with an affinity constant of 378 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
