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Human TRAIL R1 / DR4 / TNFRSF10A Protein, His Tag

Human TRAIL R1 / DR4 / TNFRSF10A Protein, His Tag
别名: TNFRSF10A,TRAIL-R1,CD261,APO2,DR4
储存条件: -20℃ 
Human TRAIL R1 / DR4 / TNFRSF10A Protein, His Tag
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-TR1-H5228-100ug 100ug 现货 2600.00 2600
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熔点:
密度:
储存条件: -20℃

Human TRAIL R1 / DR4 / TNFRSF10A Protein, His Tag

子别名(Synonym)

TNFRSF10A,TRAIL-R1,CD261,APO2,DR4

表达区间及表达系统(Source)

Human TRAIL R1, His Tag is expressed from human 293 cells  It contains AA Ala 24 - Asn 239 .

Predicted N-terminus: Ala 24

Request for sequence

蛋白结构(Molecular Characterization)

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 24.8 kDa. The protein migrates as 20-23 kDa under reducing (R) condition (SDS-PAGE).

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

Human TRAIL R1, His Tag (Cat. No. TR1-H5228) SDS-PAGE gel

Human TRAIL R1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

获取HPLC数据

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human TRAIL R1, His Tag at 5 μg/mL (100 μL/well) can bind Human TRAIL, Mouse IgG2a Fc Tag  with a linear range of 2-63 ng/mL (QC tested).

Protocol

活性(Bioactivity)-BLI

Biotinylated Human BLI

Loaded Human TRAIL R1, His Tag on HIS1K Biosensor, can bind Human TRAIL with an affinity constant of 0.583 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

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