Rat IGF-I R / CD221 Protein, His Tag

Rat IGF-I R / CD221 Protein, His Tag

分子别名（Synonym）

IGF1R,IGFR,JTK13,CD221,MGC142170,MGC142172,MGC18216

表达区间及表达系统（Source）

Rat IGF-I R, His Tagis expressed from human 293 cells. It contains AA Glu 31 - His 936.

Predicted N-terminus: Glu 31 & Asp 742

Request for sequence

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus. This protein contains a furin convertase cleavage site, 738-RRRR-741, and will be partially processed into N (α chain) and C-terminal fragment (partial β chain) with calculated MW of 81.3 kDa and 24.2 kDa respectively. The protein migrates as 45-50 kDa (partial β chain), 96-115 kDa (α chain) and 120 kDa (α chain & partial β chain) under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>90% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;

2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳（SDS-PAGE）

Rat IGF-I R, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

获取HPLC数据

 

活性（Bioactivity）-ELISA

Immobilized Rat IGF-I R, His Tag t 5 μg/mL (100 μL/well) can bind Human IGF-I, Fc Tag  with a linear range of 0.01-0.313 μg/mL (QC tested).

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