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别名: | TNFRSF1B,CD120b,TBPII,TNF-R-II,TNF-R75,TNFBR,TNFR1B,TNFR2,TNFR80,p75,p75TNFR | ||
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储存条件: | -20℃ |
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储存条件: | -20℃ |
Cynomolgus / Rhesus macaque TNFR2 / CD120b / TNFRSF1B Protein, His Tag
TNFRSF1B,CD120b,TBPII,TNF-R-II,TNF-R75,TNFBR,TNFR1B,TNFR2,TNFR80,p75,p75TNFR
Cynomolgus / Rhesus macaque TNFR2, His Tag is expressed from human 293 cells . It contains AA Leu 23 - Asp 257.
Predicted N-terminus: Leu 23
Request for sequence
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 26.9 kDa. The protein migrates as 40-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Cynomolgus / Rhesus macaque TNFR2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Biotinylated Human TNF-alpha, epitope tag free, primary amine labeling at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Cynomolgus / Rhesus macaque TNFR2, His Tagwith a linear range of 0.6-10 ng/mL (QC tested).
Protocol
Immobilized Cynomolgus / Rhesus macaque TNFR2, His Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Human TNF-alpha, epitope tag free, primary amine labelingwith a linear range of 0.3-10 ng/mL (Routinely tested).
Protocol
Immobilized Cynomolgus / Rhesus macaque TNFR2, His Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Human TNF-alpha, His,Avitag (active trimer) (MALS verified)with a linear range of 0.2-10 ng/mL (Routinely tested).