Anti-PTPRC antibody [C11-C10]

Anti-PTPRC antibody [C11-C10]

• 概述

• 产品描述CD45 has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45 are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5 and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45, while the intracellular domain is conserved. CD45 functions as a phosphotyrosine phosphatase, a vital component for efficient tyrosine phosphorylation induction by the TCR/CD3 complex. The tyrosine phosphatase activity of CD45 is contained within the conserved intracellular domain. Src and Syk family protein tyrosine kinases are utilized by the TCR/CD3 complex to initiate signaling cascades. Several members of these two families, including Lck, Fyn and ZAP-70, have been implicated as physiological substrates of CD45.

• 产品名称Anti-PTPRC antibody [C11-C10]

• 分子量147 kDa

• 种属反应性Human

• 验证应用WB,IHC-P

• 抗体类型小鼠单抗

• 免疫原Recombinant protein

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度2 mg/mL.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

• 亚型IgG1

• 纯化方式Protein A purified.

• 亚细胞定位Membrane.

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• ​其它名称

  more

  • B220 antibody

  • CD 45 antibody

  • CD45 antibody

• 应用

  WB: 1:500-1:2,000
  IHC-P: 1:100-1:200

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  Fig1: Western blot analysis of PTPRC on human PTPRC recombinant protein using anti-PTPRC antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of PTPRC on HEK293 (1) and PTPRC-hIgGFc transfected HEK293 (2) cell lysate using anti-PTPRC antibody at 1/1,000 dilution.

   

  

  Fig3: Western blot analysis of PTPRC on Hela and A431 cell lysate using anti-PTPRC antibody at 1/1,000 dilution.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PTPRC antibody. Counter stained with hematoxylin.

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