Anti-CBX2 antibody [C11-B10]

Anti-CBX2 antibody [C11-B10]

• 概述

• 产品描述Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Involved in sexual development, acting as activator of NR5A1 expression.
• 产品名称Anti-CBX2 antibody [C11-B10]
• 分子量56 kDa
• 种属反应性Human,Mouse
• 验证应用WB,ICC,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Nucleus
• 其它名称
  • Cbx 2 antibody
  • CBX2 antibody
  • CBX2_HUMAN antibody
  more

• 应用

  WB: 1:500-1:2,000
  ICC: 1:200-1:1,000
  IHC-P: 1:200-1:500
  FC: 1:50-1:200

•  

  

  Fig1: Western blot analysis of CBX2 on human CBX2 recombinant protein using anti-CBX2 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of CBX2 on HEK293 (1) and CBX2-hIgGFc transfected HEK293 (2) cell lysate using anti-CBX2 antibody at 1/1,000 dilution.

   

  

  Fig3: Western blot analysis of CBX2 on different cell lysate using anti-CBX2 antibody at 1/1,000 dilution.
  Positive control: Line1: HUVEC Line2: HEK293 Line3: Hela Line4: NIH/3T3 Line5: A431

   

  

  Fig4: ICC staining CBX2 (green) and Actin filaments (red) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues using anti-CBX2 antibody. Counter stained with hematoxylin.

   

  

  Fig6: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using anti-CBX2 antibody. Counter stained with hematoxylin.

   

  

  Fig7: Flow cytometric analysis of Hela cells with CBX2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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