Anti-ZEB1 antibody [A2-D8]

Anti-ZEB1 antibody [A2-D8]

• 概述

• 产品描述ZEB1 is a non-receptor transcription factor analogous to the Drosophila ZFH-1 protein. ZEB1 contains two separate zinc finger domains (ZD1 and ZD2), which are essential for DNA binding and repression, and a homeodomain, which is not. ZEB1 also contains three repression domains, two of which flank ZD1, and a third located between HD and ZD2. ZEB1 represses transcription by site competition and enhancer silencing mechanisms, as well as by interacting with corepressors through its repression domains. Interaction of ZEB1 with the TSHβ gene T3-response element may play a role in the modification of gene-specific regulation by thyroid hormones. In the embryo, ZEB1 is primarily expressed in the mesoderm, but changes in the level of expression during tissue maturation suggest a role for ZEB1 in the early histogenesis of mesodermal tissues.
• 产品名称Anti-ZEB1 antibody [A2-D8]
• 分子量124 kDa
• 种属反应性Human
• 验证应用WB,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Nucleus.
• 其它名称
  • AREB 6 antibody
  • AREB6 antibody
  • BZP antibody
  more

• 应用

  WB: 1:500-1:1,000
  IHC-P: 1:100-1:500
  FC: 1:100-1:200

•  

  

  Fig1: Western blot analysis of ZEB1 on human ZEB1 recombinant protein using anti-ZEB1 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of ZEB1 on HEK293 (1) and ZEB1-hIgGFc transfected HEK293 (2) cell lysate using anti-ZEB1 antibody at 1/1,000 dilution.

   

  

  Fig3: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-ZEB1 antibody. Counter stained with hematoxylin.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-ZEB1 antibody. Counter stained with hematoxylin.

   

  

  Fig5: Flow cytometric analysis of Hela cells with ZEB1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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