Anti-CD64 antibody [5F5F8]

Anti-CD64 antibody [5F5F8]

• 概述

• 产品描述This gene encodes a protein that plays an important role in the immune response. This protein is a high-affinity Fc-gamma receptor. The gene is one of three related gene family members located on chromosome 1.
• 产品名称Anti-CD64 antibody [5F5F8]
• 分子量42.6kDa
• 种属反应性Human
• 验证应用WB,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Purified recombinant fragment of human CD64 (AA: extra 16-145) expressed in E. Coli.
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS with 0.05% sodium azide.
• 亚型IgG1
• 纯化方式Protein G purified.
• 亚细胞定位Cell membrane. Stabilized at the cell membrane through interaction with FCER1G.
• 其它名称
  • CD 64 antibody
  • CD64 antibody
  • CD64 antigen antibody
  more

• 应用

  WB: 1:500-1:2,000
  IHC-P: 1:50-1:200
  FC: 1:100-1:200

•  

  

  Fig1: Western blot analysis of CD64 against human CD64 (AA: extra 16-145) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

   

  

  Fig2: Western blot analysis of EM1710-99 against HEK293 (1) and CD64 (AA: extra 16-145)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

   

  

  Fig3: Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-CD64 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody  for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

   

  

  Fig4: Flow cytometric analysis of CD64 was done on HL-60 cells. The cells were fixed, permeabilized and stained with the primary antibody (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！