Anti-AFAP1L2 antibody [D1-H6]

Anti-AFAP1L2 antibody [D1-H6]

• 概述

• 产品描述Actin filament associated protein (AFAP-110) interacts directly with actin filaments through its C-terminal actin-binding domain. AFAP-110 contains additional protein-binding domains as well, and serves as an adaptor protein. By linking signaling molecules to actin filaments, AFAP-110 provides a platform for the preparation of larger signaling complexes, activates Src kinases in response to cellular signals and also directly affects Actin organization as an Actin filament cross-linking protein. AFAP-1L2 (Actin filament-associated protein 1-like 2), also known as XB130, is a 818 amino acid cytoplasmic protein that contains two Pleckstrin homology (PH) domains, which are normally found in proteins involved in intracellular signaling. Like its relative AFAP110, AFAP-1L2 interacts with Src kinase and may play a role in Src-regulated transcription activation. AFAP-1L2 is expressed in thyroid and spleen and can also be detected at lower levels in lung, brain, pancreas and kidney. There are four isoforms of AFAP-1L2 that are produced as a result of alternative splicing events
• 产品名称Anti-AFAP1L2 antibody [D1-H6]
• 分子量91 kDa
• 种属反应性Human
• 验证应用WB,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Cytoplasm.
• 其它名称
  • Actin filament-associated protein 1-like 2 antibody
  • AF1L2_HUMAN antibody
  • AFAP1-like protein 2 antibody
  more

• 应用

  WB: 1:500-1:2,000
  FC: 1:100-1:200

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  Fig1: Western blot analysis of AFAP1L2 on human AFAP1L2 recombinant protein using anti-AFAP1L2 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of AFAP1L2 on HEK293 (1) and AFAP1L2-hIgGFc transfected HEK293 (2) cell lysate using anti-AFAP1L2 antibody at 1/1,000 dilution.

   

  

  Fig3: Western blot analysis of AFAP1L2 on A549 cell lysate using anti-AFAP1L2 antibody at 1/1,000 dilution.

   

  

  Fig4: Flow cytometric analysis of Hela cells with AFAP1L2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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