Anti-GABA Transporter 3 antibody

Anti-GABA Transporter 3 antibody

• 概述

• 产品描述Terminates the action of GABA by its high affinity sodium-dependent reuptake into presynaptic terminals.

• 产品名称Anti-GABA Transporter 3 antibody

• 分子量71 KDa

• 种属反应性Human,Mouse,Rat

• 验证应用WB,FC

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human GABA Transporter 3/GAT 3 531-632/632

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Membrane; Multi-pass membrane protein.

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• ​其它名称GABT3
  GAT-3
  GAT3
  S6A11_HUMAN
  Slc6a11
  Sodium- and chloride-dependent GABA transporter 3
  SOLUTE CARRIER FAMILY 6 (NEUROTRANSMITTER TRANSPORTER, GABA), MEMBER 11
  Solute carrier family 6 member 11.

  more

• 应用

  WB:1:500-2000
  FC:1ug/test

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  Fig1: Sample:A431 (human)cell Lysate at 40 ug
  Primary: Anti-GABA Transporter 3 at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
  Predicted band size: 71 kD
  Observed band size: 71 kD

   

  

  Fig2: Blank control: HepG2.
  Primary Antibody (green line): Rabbit Anti-GABA Transporter 3 antibody 
  Dilution: 1μg /10^6 cells;
  Isotype Control Antibody (orange line): Rabbit IgG .
  Secondary Antibody : Goat anti-rabbit IgG-PE
  Dilution: 1μg /test.
  Protocol
  The cells then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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