Anti-CD73 antibody [6E-G3]

Anti-CD73 antibody [6E-G3]

• 概述

• 产品描述CD73 (also designated ecto-5'-nucleotidase, E5NT, NT, NT5, NTE, eN and eNT) is a glycosyl-phosphatidylinositol (GPI)-anchored adhesion protein that catalyzes the dephosphorylation of extracellular purine and pyrimidine nucleotides to their corresponding bioactive nucleosides. CD73 is a dimer of two identical subunits that depends on GPI to link with the external face of the plasma membrane. Similar to other GPI-anchored proteins, CD73 mediates co-stimulatory signals in T cell activation. CD73 has few structural variants, yet elicits diverse biological function through differential regulation in endothelial cells (EC), subpopulations of B and T cells, germinal center follicular dendritic cells and on thymic medullary reticular fibroblasts. For example, IgG mediated neutralization of CD73 interferes with lymphocyte adhesion to EC, and blocks aggregation of germinal center B cells and follicular dendritic cells. Furthermore, IgG-mediated targeting of lymphocyte CD73, but not of endothelial cell CD73, causes shedding of CD73 and tyrosine phosphorylation of proteins.
• 产品名称Anti-CD73 antibody [6E-G3]
• 分子量63 kDa
• 种属反应性Human
• 验证应用WB,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Cell membrane.
• 其它名称
  • 5' NT antibody
  • 5' nucleotidase (CD73) antibody
  • 5' nucleotidase precursor antibody
  more

• 应用

  WB: 1:500-1:2,000
  FC: 1:50-1:200
  IHC-P: 1:50-1:200

•  

  

  Fig1: Western blot analysis of CD73 on human CD73 recombinant protein using anti-CD73 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of CD73 on HEK293 (1) and RAN-hIgGFc transfected HEK293 (2) cell lysate using anti- CD73 antibody at 1/1,000 dilution.

   

  

  Fig3: Western blot analysis of CD73 on A431 cell lysate using anti- CD73 antibody at 1/1,000 dilution.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue using anti- CD73 antibody. Counter stained with hematoxylin.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti- CD73 antibody. Counter stained with hematoxylin.

   

  

  Fig6: Flow cytometric analysis of HepG2 cells with CD73 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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