Anti-HSP40 antibody [3G2]

Anti-HSP40 antibody [3G2]

• 概述

• 产品描述his gene encodes a member of the DnaJ or Hsp40 (heat shock protein 40 kD) family of proteins. DNAJ family members are characterized by a highly conserved amino acid stretch called the 'J-domain' and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. The encoded protein is a molecular chaperone that stimulates the ATPase activity of Hsp70 heat-shock proteins in order to promote protein folding and prevent misfolded protein aggregation. Alternative splicing results in multiple transcript variants.
• 产品名称Anti-HSP40 antibody [3G2]
• 分子量38 KDa
• 种属反应性Human,Mouse,Rat
• 验证应用WB
• 抗体类型小鼠单抗
• 免疫原Recombinant human HSP40 Protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• 亚型IgG1
• 纯化方式affinity purified by Protein A
• 亚细胞定位Cytoplasm. Nucleus. Nucleus > nucleolus. Translocates rapidly from the cytoplasm to the nucleus, and especially to the nucleoli, upon heat shock.
  
• 其它名称
  • HSP40 homolog
  • DnaJ (Hsp40) homolog subfmaily B member 1
  • DNAJ 1
  more

• 应用

  WB:1:500-2000

•  

  

  Fig1: Sample:
  Hela(Human) Cell Lysate at 30 ug
  Primary: Anti- HSP40 at 1/1000 dilution
  Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
  Predicted band size: 38 kD
  Observed band size: 38 kD

   

  

  Fig2: Sample:
  U251(Human) Cell Lysate at 30 ug
  A549(Human) Cell Lysate at 30 ug
  Primary: Anti- HSP40at 1/1000 dilution
  Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
  Predicted band size: 38 kD
  Observed band size: 38 kD

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