Anti-CD3EAP antibody
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概述
- 产品描述caution:It is not known whether the so-called human ASE1 and human CAST proteins represent two sides of a single gene product with sharply different functional characteristics. Experiments done with the mouse homolog protein are in favor of an implication of this gene in rRNA transcription instead of T-cell receptor signaling. DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Isoform 1 is involved in UBTF-activated transcription, presumably at a step following PIC formation. Isoform 2 has been described as a component of preformed T-cell receptor (TCR) complex. miscellaneous:It is in an antisense orientation to and overlaps the gene of the DNA repair enzyme ERCC1. This gene overlap is conserved in mouse suggesting an important biologic function. PTM:Isoform 1 is phosphorylated on tyrosine residues in initiation-competent Pol I-beta complexes but not in Pol I-alpha complexes. PTM:Isoform 2 undergoes tyrosine phosphorylation upon T-cell receptor (TCR) stimulation. This phosphorylation has not been confirmed by other group. PTM:Phosphorylated upon DNA damage, probably by ATM or ATR. Belongs to the eukaryotic RPA34 RNA polymerase subunit family. subcellular location:Found at the fibrillar centers of the nucleolus in interphase and during cell division it is localized to the nucleolus organizer regions of the chromosomes. subunit:Component of the RNA polymerase I (Pol I) complex consisting of at least 13 subunits. Interacts with TAF1A thereby associates with the SL1 complex. Interacts with UBTF. Interacts with POLR1E/PRAF1 through its N-terminal region (By similarity). Isoform 2 interacts with CD3E.
- 产品名称Anti-CD3EAP antibody
- 分子量55 kDa
- 种属反应性Human
- 验证应用WB, IHC-P
- 抗体类型兔多抗
- 免疫原The antiserum was produced against synthesized peptide derived from human CD3EAP. AA range:441-490
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 亚型IgG
- 纯化方式Immunogen affinity purified.
- 其它名称CD3EAP antibody; ASE1 antibody; CAST antibody; PAF49 antibody; DNA-directed RNA polymerase I subunit RPA34 antibody; A34.5 antibody; Antisense to ERCC-1 protein antibody; ASE-1 antibody; CD3-epsilon-associated protein antibody; CAST antibody; CD3E-associated protein antibody; RNA polymerase I-associated factor PAF49 antibody;
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应用
WB: 1:500-1:2000
IHC-P: 1:100-1:300
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Fig1: Western Blot analysis of A549 cells using CD3EAP Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
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