Anti-MCM2 antibody [C5-E7]

Anti-MCM2 antibody [C5-E7]

• 概述

• 产品描述The mini-chromosome maintenance (MCM) family of proteins, including MCM2, MCM3, MCM4 (Cdc21), MCM5 (Cdc46), MCM6 (Mis5) and MCM7 (Cdc47), are regulators of DNA replication that act to ensure replication occurs only once in the cell cycle. Expression of MCM proteins increases during cell growth, peaking at G1 to S phase. The MCM proteins each contain an ATP-binding motif, which is predicted to mediate ATP-dependent opening of double-stranded DNA. MCM proteins are regulated by E2F transcription factors, which induce MCM expression, and by protein kinases, which interact with MCM proteins to maintain the post-replicative state of the cell. MCM2/MCM4 complexes function as substrates for Cdc2/cyclin B in vitro. Cleavage of MCM3, which can be prevented by caspase inhibitors, results in the inactivation during apoptosis of the MCM complex, which is composed of, at least, MCM2–6. A complex composed of MCM4, MCM6 and MCM7 has been shown to be involved in DNA helicase activity, and MCM5 is involved in IFN-γ-induced Stat1α transcription activation.

• 产品名称Anti-MCM2 antibody [C5-E7]

• 分子量102 kDa

• 种属反应性Human

• 验证应用WB,ICC,IHC-P,FC

• 抗体类型小鼠单抗

• 免疫原Recombinant protein

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度2 mg/mL.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.

• 亚型IgG1

• 纯化方式Protein A purified.

• 亚细胞定位Nucleus.

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• ​其它名称

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  • BM28 antibody

  • CCNL 1 antibody

  • CCNL1 antibody

• 应用

  WB: 1:500-1:1,000
  ICC: 1:100-1:500
  IHC-P: 1:100-1:500
  FC: 1:100-1:200

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  Fig1: Western blot analysis of MCM2 on human MCM2 recombinant protein using anti-MCM2 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of MCM2 on different cell lysates using anti-MCM2 antibody at 1/1,000 dilution.
  Positive control:
  Lane 1: MCF-7
  Lane 2: Hela
  Lane 3: Jurkat Lane
  4: K562
  Lane 5: HEK293
  Lane 6:HepG2

   

  

  Fig3: ICC staining MCM2 (green) and actin filaments (red) in Hela cells. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissues using anti-MCM2 antibody. Counter stained with hematoxylin.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer using anti-MCM2 antibody. Counter stained with hematoxylin.

   

  

  Fig6: Flow cytometric analysis of Jurkat cells with MCM2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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