Anti-HIV1 p24 antibody [12G4]

Anti-HIV1 p24 antibody [12G4]

• 概述

• 产品描述One distinctive HIV antigen is a viral protein called p24, a structural protein that makes up most of the HIV viral core, or 'capsid'. There are approximately 2000 molecules per virus particle, or at a molecule weight of 24 kDa, about 104 virus particles per picogram of p24. The onset of symptoms of AIDS correlates with a reduction in the number of CD4+ T-cells and increased levels of virus and p24 in the blood. It is a component of the gag polyprotein. High levels of p24 are present in the blood serum of newly infected individuals during the short period between infection and seroconversion, making p24 antigen assays useful in diagnosing primary HIV infection. Antibodies to p24 are produced during seroconversion, rendering p24 antigen undetectable after seroconversion in most cases. Therefore, p24 antigen assays are not reliable for diagnosing HIV infection after its very earliest stages. Fourth-generation HIV immunoassays detect viral p24 protein in the blood (as well as patient antibodies against the virus). Previous generation tests relied on detecting patient antibodies alone; it takes about 3–4 weeks for the earliest antibodies to be detected. The p24 protein can be detected in patient blood as early as 2 weeks after HIV infection, further reducing the window period necessary to accurately detect the HIV status of the patient.
• 产品名称Anti-HIV1 p24 antibody [12G4]
• 分子量24 kDa
• 种属反应性HIV1
• 验证应用ELISA(Cap), WB
• 抗体类型小鼠单抗
• 免疫原Recombinant protein within HIV1 p24 protein aa 133-363 (Catalog#HA210278).
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein G affinity purified.
• 亚细胞定位Virion.
• 其它名称
  • CA antibody
  • Capsid protein p24 antibody
  • HIV-1 Gag p24 antibody
  more

• 应用

  ELISA: 5-10 ng/ml
  WB: 1,000-1:5,000

•  

  

  Fig1: The binding activity of HA600010 with HIV1 p24 protein.
  Immobilized HIV1 p24 protein at 1 μg/ml overnight at 4℃. Then blocked with 1% BSA for 1 hour at 37℃, and incubated with the primary antibody (HA600010) for 1 hour at 25℃. The EC50 of HA600010 is 579 ng/ml.

   

  

  Fig2: This antibody will detect HIV1 p24 protein in ELISA pair set (Cat: # HA600010). In a sandwich ELISA, it can be used as Capture antibody when paired with (Cat: # HA600009).

   

  

  Fig3: Western blot using anti-HIV1 p24 protein antibody shows detection of a 25 kDa band corresponding to Recombanint HIV1 p24 protein.

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