Anti-JAK1 antibody [1G1]

Anti-JAK1 antibody [1G1]

• 概述

• 产品描述Jak1 (Janus kinase 1) belongs to the family of non-receptor janus tyrosine kinases, which regulate a spectrum of cellular functions downstream of activated cytokine receptors in the lympho-hematopoietic system. Immunological stimuli, such as interferons and cytokines, induce recruitment of STAT transcription factors to cytokine receptor-associated Jak1. Jak1 then phosphorylates proximal STAT factors, which subsequently dimerize, translocate to the nucleus and bind to cis elements upstream of target gene promoters to regulate transcription. Upon ligand binding, Jak1 undergoes tyrosine phosphorylation and catalytic activation in an interdependent manner. Phosphorylation of tyrosine residues at position 1022 and 1023 is believed to function in the activation of catalytic events. The canonical Jak-STAT pathway is integral to maintaining a normal immune system by stimulating proliferation, differentiation, survival, and host resistance to pathogens. Altering Jak-STAT signaling to reduce cytokine induced pro-inflammatory responses represents an attractive target for anti-inflammatory therapies.
• 产品名称Anti-JAK1 antibody [1G1]
• 分子量130kDa
• 种属反应性Human,Mouse,Rat
• 验证应用WB,IP,IF,IHC-P
• 抗体类型小鼠单抗
• 免疫原Amino acids 785-815 within an internal region of JAK1 of mouse origin.
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Endomembrane system
• 其它名称
  • JAK 1 antibody
  • JAK 1A antibody
  • JAK 1B antibody
  more

• 应用

  WB: 1:100-1:1,000
  IP: 1-2 μg per 100-500 μg of total protein(1 ml of cell lysate)
  IF: 1:50-1:500
  IHC-P: 1:50-1:500

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  Fig1: Western blot analysis of JAK1 expression in non-transfected (A) and truncated mouse JAK1 transfected (B) 293T whole cell lysates.

   

  

  Fig2: Immunofluorescence staining of methanol-fixed Jurkat cells showing membrane localization (A). Immunoperoxidase staining of formalin fixed, paraffin-embedded Jurkat showing membrane and cytoplasmic localization (B).

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