Anti-THAP11 antibody [A4-F3]

Anti-THAP11 antibody [A4-F3]

• 概述

• 产品描述Members of the THAP (thanatos-associated protein) family of proteins contain a well conserved DNA-binding domain known as the THAP-type zinc finger motif. Proteins containing the THAP-type zinc finger motif are commonly involved in transcriptional regulation, cell-cycle control, apoptosis and chromatin modification. The THAP-type zinc finger domain is suggested to have similarities with the site-specific DNA-binding domain (DBD) of Drosophila P element transposase. THAP11 (THAP domain containing 11), also known as HRIHFB2206, is a 314 amino acid protein that belongs to the THAP11 family and contains one THAP-type zinc finger. Localizing to the nucleus and cytoplasm, THAP11 may act as a transcriptional repressor, playing a role in embryogenesis and pluripotency of embryonic stem cells by recruiting epigenetic modifiers. THAP11 interacts with HCF1 via a coiled coil domain.
• 产品名称Anti-THAP11 antibody [A4-F3]
• 分子量45kDa,additional band 75kDa
• 种属反应性Human
• 验证应用WB,ICC,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Nucleus. Cytoplasm.
• 其它名称
  • CTG B43a antibody
  • CTG B45d antibody
  • HRIHFB2206 antibody
  more

• 应用

  WB: 1:500
  ICC: 1:100-1:500
  IHC-P: 1:50-1:200
  FC: 1:100-1:200

•  

  

  Fig1: Western blot analysis of THAP11 on Hela (1) and NTERA-2 (2) cell lysate using anti-THAP11 antibody at 1/1,000 dilution.

   

  

  Fig2: ICC staining THAP11 (green) and actin filaments (red) in NTERA-2 cells. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig3: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-THAP11 antibody. Counter stained with hematoxylin.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue using anti-THAP11 antibody. Counter stained with hematoxylin.

   

  

  Fig5: Flow cytometric analysis of Hela cells with THAP11 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).

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