Anti-BOB1 antibody
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概述
- 产品描述Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers. Reduced activation of Bob1-deficient B cells following antigenic stimulation is often reported. The action of Bob1 on B cell maturation and tolerance could be mediated by effects on B cell activation, in part by regulating the expression of immunosuppressive miRNAs. Bob1 has long been considered a B cell‐specific factor that interacts with the transcription factors Oct1 and Oct2 to enhance octamer‐dependent transcription. Bob1 can directly bind to and transactivate the promoters of Bcl6 and Btla.
- 产品名称Anti-BOB1 antibody
- 分子量35 kDa (predicted molecular weight: 27 kDa)
- 种属反应性Human,Mouse,Rat
- 验证应用WB,FC
- 抗体类型兔多抗
- 免疫原Recombinant protein within Human BOB1 aa 10-180.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/ml.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Protein affinity purified.
- 亚细胞定位Nucleus.
- 其它名称B cell Oct binding protein 1 antibody
B cell specific coactivator OBF 1 antibody
B cell specific coactivator OBF1 antibody
B-cell-specific coactivator OBF-1 antibody
BOB 1 antibody
BOB-1 antibody
OBF 1 antibody
OBF1 antibody
OBF1_HUMAN antibody
OCA B antibody
OCA-B antibody
OCAB antibody
OCT binding factor 1 antibody
OCT-binding factor 1 antibody
POU class 2 associating factor 1 antibody
POU domain class 2 associating factor 1 antibody
POU domain class 2-associating factor 1 antibody
Pou2af1 antibody
more
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应用
WB:1:500
FC:1:50-1:100
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Fig1: Western blot analysis of BOB1 on Daudi lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Fig2: Flow cytometric analysis of BOB1 was done on Daudi cells. The cells were fixed, permeabilized and stained with BOB1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). After incubation of the primary antibody on room temperature for an hour, the cells was stained with a Alexa Fluor™ 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.
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